AB0001 ALLELIC POLYMORPHISM OF INFLAMMATORY CYTOKINES GENES FOR PREDICTION OF CARDIOVASCULAR DISORDERS IN CHILDREN WITH JIA

Background It is known that defects in the synthesis and structure of cytokines or their receptors can lead to modification many pathological processes organism: inflammation, coagulation, impaired cell regeneration, which makes it appropriate further study genetic preconditions for violation immunoregulatory mechanisms. Great attention paid allelic polymorphism genes chronic inflammatory diseases as a potential marker functional activity proteins encoded by them related features course various disease. Objectives To assess impact TNFα (G308A) & IL6 (G174C) on development cardiovascular disorders children with JIA. Methods 102 JIA were examined. Allelic (G308A)& was determined PCR using specific oligonucleotide primers, subsequent analysis restriction fragments made. Activity disease calculated JADAS27. Functional state myocardium assessed software-hardware complex “Cardioplus P”, an ECG 4 th generation. Cluster obtained data performed. K-means EM algorithms used. The number who finally entered this stage 25. Results According activity, mutations indicator (CI FS, %), two clusters identified. In cluster 1 (10 children, group 1) higher score CI FS (median 78%) lower degree (JADAS 27 median 9.5) noted, 2 (15 2) high 14) observed decrease 62%). according generation differed significantly ( p = 0.00001) clusters. noted second debuted relatively earlier (p>0.01). Among these groups patients there significant differences general clinical laboratory parameters: AST blood 29±3.1 IU/ l first vs 24±2.2 IU/l (p 0.07 Wald-Wolfowitz), serum creatinine 68.8±11.0 μmol/l (against 57.0±9.3 group; t -2.24, 0.044), also results leukocytosis (11.6±4.1 x10 9 vs. 6.69±0.8 , 0.075 Wald-Wolfowitz). analysis, total TNF-α cases larger mutations, more aggressive disease, pronounced frequency extra-articular lesions, including changes CVS, noted. Conclusion overall disease; are associated lesions likely. Determination should be recommended have performed 4th ECG. Disclosure Interests None declared